Argentina, Brasil, Chile, Colombia, España, Guatemala, Mexico, Perú, Portugal
Artículo Revista Iberoamericana de Micología
Tipo de Publicación:
Microbiología y biología molecular
Background: The fungi present in the decaying remains enable a better understanding of the processesof decomposition after death. There are not many studies about fungi on decaying bodies and it is notknown which fungal sampling methods are effective.Aims: The main objective of this study was to find the best method for sampling fungi in carcasses, provethe effectiveness of this method and identify the fungal colonies in animal carcasses from experimentalburials.Methods: Samples from 13 carcasses of Sus scrofa domestica, from the experimental project Taphos-m,were taken with different materials: spatula, sterile swabs and RODAC contact plates.Results: RODAC contact plates with the RBA culture medium showed higher proliferation of fungalcolonies. Thirty genera of fungi were isolated from different substrates (bone, tissue, lime). Most of thefungi genera or groups identified have been described before in the literature, but the substrates theycame from were different in some cases.Conclusions: Sampling with RODAC contact plates was found to be the most effective method, as it pro-vides a nutritional culture medium that may allow growth since the moment of sampling. Fungi coloniesgrew better in RBA culture medium because bacterial growth is inhibited. Most of the observed fungi are related to the environment but some others have been found related to decomposing bodies for the first time.